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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCUN1D1 Recombinant antibody (85849-4-RR)

    WB analysis using 85849-4-RR (same clone as 85849-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85849-4-RR (DCUN1D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85849-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85849-4-RR

    Affinity and Kinetic Characterization of 85849-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85849-4-RR against Human DCUN1D1 were performed. The affinity constant is 23.8 pM.

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DCUN1D1 Recombinant antibody, PBS Only

DCUN1D1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85849-4-PBS
Clone No.250196B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

DCN1, DCN1 like protein 1, DCN1-like protein 1, DCNL1, DCUN 1D1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85849-4-PBS targets DCUN1D1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DCUN1D1 fusion protein Ag1440 相同性解析による交差性が予測される生物種
Full Name DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009478
Gene Symbol DCUN1D1
Gene ID (NCBI) 54165
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96GG9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

It has been found that DCUN1D1, also known as squamous cell carcinoma-related oncogene, expression may play a role in development of brain metastasis in patients with Non-small cell lung carcinoma (NSCLC). DCUN1D1-positive tumor cells may have the ability to disrupt the blood-brain-barrier and colonize the brain.