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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DCTPP1 Polyclonal antibody (16684-1-AP)

    WB analysis of HeLa using 16684-1-AP (same clone as 16684-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16684-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using DCTPP1 Polyclonal antibody (16684-1-AP)

    WB analysis of mouse heart using 16684-1-AP (same clone as 16684-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16684-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using DCTPP1 Polyclonal antibody (16684-1-AP)

    WB analysis of Transfected HEK-293 using 16684-1-AP (same clone as 16684-1-PBS)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DCTPP1 Polyclonal antibody (16684-1-AP)

    IHC staining of human lung cancer using 16684-1-AP (same clone as 16684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DCTPP1 Polyclonal antibody (16684-1-AP)

    IHC staining of human lung cancer using 16684-1-AP (same clone as 16684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16684-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DCTPP1 Polyclonal antibody (16684-1-AP)

    IF Staining of HeLa using 16684-1-AP (same clone as 16684-1-PBS)

    Immunofluorescent analysis of HeLa cells using 16684-1-AP (DCTPP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16684-1-PBS in a different storage buffer formulation.

DCTPP1 Polyclonal antibody, PBS Only

DCTPP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16684-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16684-1-PBS targets DCTPP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10015

Product name: Recombinant human DCTPP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-170 aa of BC001344

Sequence: MSVAGGEIRGDTGGEDTAAPGRFSFSPEPTLEDIRRLHAEFAAERDWEQFHQPRNLLLALVGEVGELAELFQWKTDGEPGPQGWSPRERAALQEELSDVLIYLVALAARCRVDLPLAVLSKMDINRRRYPAHLARSSSRKYTELPHGAISEDQAVGPADIPCDSTGQTST

相同性解析による交差性が予測される生物種
Full Name dCTP pyrophosphatase 1
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa, 40 kDa, 80 kDa
GenBank accession numberBC001344
Gene Symbol DCTPP1
Gene ID (NCBI) 79077
RRIDAB_2878299
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H773
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCTPP1, also named as XTP3TPA, CDAO3 and RS21C6, is a hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. It has a central role in the balance of dCTP and the metabolism of deoxycytidine analogues, thus contributing to the preservation of genome integrity. DCTPP1 has some forms such as monomer (~20 kDa), Dimer (~40 kDa) and Homotetramer (~80 kDa).

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