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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCTN3 Polyclonal antibody (11325-1-AP)

    WB analysis using 11325-1-AP (same clone as 11325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11325-1-AP (DCTN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DCTN3 Polyclonal antibody (11325-1-AP)

    IHC staining of mouse brain using 11325-1-AP (same clone as 11325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11325-1-AP (DCTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DCTN3 Polyclonal antibody (11325-1-AP)

    IHC staining of mouse brain using 11325-1-AP (same clone as 11325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11325-1-AP (DCTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DCTN3 Polyclonal antibody (11325-1-AP)

    IHC staining of rat brain using 11325-1-AP (same clone as 11325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11325-1-AP (DCTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DCTN3 Polyclonal antibody (11325-1-AP)

    IHC staining of rat brain using 11325-1-AP (same clone as 11325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11325-1-AP (DCTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11325-1-PBS in a different storage buffer formulation.

DCTN3 Polyclonal antibody, PBS Only

DCTN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11325-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DCTN 22, DCTN 3, DCTN22, dynactin 3 (p22), p22

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11325-1-PBS targets DCTN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1865

Product name: Recombinant human DCTN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC000319

Sequence: MAGLTDLQRLQARVEELERWVYGPGGARGSRKVADGLVKVQVALGNISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQAPWGVGVRDEAGSLVEDVGFAQFLSVLHFGPTGPVCGNH

相同性解析による交差性が予測される生物種
Full Name dynactin 3 (p22)
Calculated molecular weight 186 aa, 21 kDa
Observed molecular weight22 kDa
GenBank accession numberBC000319
Gene Symbol DCTN3
Gene ID (NCBI) 11258
RRIDAB_3741816
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75935
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCTN3 (Dynactin Subunit 3, also known as p22) is a core component of the dynactin complex, essential for cytoplasmic dynein-mediated intracellular transport. This 22-kDa polypeptide binds directly to microtubules and stabilizes the dynactin structure, facilitating cargo movement along microtubule tracks. DCTN3 plays critical roles in mitotic spindle organization, vesicle trafficking, and neuronal development (PMID: 24352458).

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