Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
13095-1-AP targets DCT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DCT fusion protein Ag3714 相同性解析による交差性が予測される生物種 |
| Full Name | dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) |
| Calculated molecular weight | 519 aa, 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC028311 |
| Gene Symbol | DCT |
| Gene ID (NCBI) | 1638 |
| RRID | AB_2090576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DCT antibody 13095-1-AP | Download protocol |
| IHC protocol for DCT antibody 13095-1-AP | Download protocol |
| IF protocol for DCT antibody 13095-1-AP | Download protocol |
| IP protocol for DCT antibody 13095-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin. | ||
Hum Mol Genet Transcriptome analysis and molecular signature of human retinal pigment epithelium. | ||
J Invest Dermatol miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes. | ||
J Cell Mol Med Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes | ||
Sci Rep Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage. | ||
Pharmaceutics Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion. |