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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCPS Polyclonal antibody (28465-1-AP)

    WB analysis using 28465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28465-1-AP (DCPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DCPS Polyclonal antibody (28465-1-AP)

    IF Staining of HeLa using 28465-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCPS antibody (28465-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

DCPS Polyclonal antibody

DCPS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28465-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.6.1.59, Decapping scavenger enzyme, decapping enzyme, scavenger, DCS-1, DCS1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28465-1-AP targets DCPS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29351

Product name: Recombinant human DCPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-160 aa of BC014532

Sequence: QLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRN

相同性解析による交差性が予測される生物種
Full Name decapping enzyme, scavenger
Calculated molecular weight 337 aa, 39 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014532
Gene Symbol DCPS
Gene ID (NCBI) 28960
RRIDAB_3086054
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96C86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

Protocols

Product Specific Protocols
IF protocol for DCPS antibody 28465-1-APDownload protocol
WB protocol for DCPS antibody 28465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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