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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCLK1 Monoclonal antibody (68234-1-Ig)

    WB analysis using 68234-1-Ig (same clone as 68234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using DCLK1 Monoclonal antibody (68234-1-Ig)

    WB analysis of fetal human brain using 68234-1-Ig (same clone as 68234-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DCLK1 Monoclonal antibody (68234-1-Ig)

    IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DCLK1 Monoclonal antibody (68234-1-Ig)

    IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DCLK1 Monoclonal antibody (68234-1-Ig)

    IF Staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (68234-1-Ig, Clone: 3C12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Neuro-2a cells using DCLK1 Monoclonal antibody (68234-1-Ig)

    FC experiment of Neuro-2a using 68234-1-Ig (same clone as 68234-1-PBS)

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human DCLK1 (68234-1-Ig, Clone:3C12C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

DCLK1 Monoclonal antibody, PBS Only

DCLK1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 68234-1-PBS
Clone No.3C12C1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A, DCAMKL1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68234-1-PBS targets DCLK1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17110

Product name: Recombinant human DCLK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 623-729 aa of BC152456

Sequence: LITMMLLVDVDQRFSAVQVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIALDHGFTIKRSGSLDYYQQPGMYWIRPPLLIRRGRFSDEDATRM

相同性解析による交差性が予測される生物種
Full Name doublecortin-like kinase 1
Calculated molecular weight 729 aa, 81 kDa
Observed molecular weight46 kDa, 82 kDa
GenBank accession numberBC152456
Gene Symbol DCLK1
Gene ID (NCBI) 9201
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO15075
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.