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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCLK1 Polyclonal antibody (29800-1-AP)

    WB analysis using 29800-1-AP (same clone as 29800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IP experiment of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

DCLK1 Polyclonal antibody, PBS Only

DCLK1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 29800-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

DCAMKL1, DCDC3A, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29800-1-PBS targets DCLK1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32007

Product name: Recombinant human DCLK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 648-740 aa of NM_004734

Sequence: NDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIATTALDKERQVFRRRRNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF

相同性解析による交差性が予測される生物種
Full Name doublecortin-like kinase 1
Calculated molecular weight82KD
Observed molecular weight48 kDa, 82 kDa
GenBank accession numberNM_004734
Gene Symbol DCLK1
Gene ID (NCBI) 9201
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15075
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.