• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DCLK1 Polyclonal antibody (29800-1-AP)

    WB analysis using 29800-1-AP (same clone as 29800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IP experiment of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DCLK1 Polyclonal antibody (29800-1-AP)

    IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

This product is currently not available for sale.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29800-1-PBS targets DCLK1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCLK1 fusion protein Ag32007 相同性解析による交差性が予測される生物種
Full Name doublecortin-like kinase 1
Calculated molecular weight82KD
Observed molecular weight48 kDa, 82 kDa
GenBank accession numberNM_004734
Gene Symbol DCLK1
Gene ID (NCBI) 9201
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15075
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.