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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCHS1 Polyclonal antibody (28144-1-AP)

    WB analysis using 28144-1-AP (same clone as 28144-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 28144-1-AP (DCHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28144-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DCHS1 Polyclonal antibody (28144-1-AP)

    IF Staining of mouse brain using 28144-1-AP (same clone as 28144-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using DCHS1 antibody (28144-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28144-1-PBS in a different storage buffer formulation.

DCHS1 Polyclonal antibody, PBS Only

DCHS1 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 28144-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

Cadherin 19, Cadherin 25, CDH19, CDH25, dachsous 1 (Drosophila)

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28144-1-PBS targets DCHS1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27226

Product name: Recombinant human DCHS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1517-1718 aa of NM_003737

Sequence: MPANASRRRAARVSARVFVTDENDNAPVFASPSRVRLPEDQPPGPAALHVVARDPDLGEAARVSYRLASGGDGHFRLHSSTGALSVVRPLDREQRAEHVLTVVASDHGSPPRSATQVLTVSVADVNDEAPTFQQQEYSVLLRENNPPGTSLLTLRATDPDVGANGQVTYGGVSSESFSLDPDTGVLTTLRALDREEQEEIN

相同性解析による交差性が予測される生物種
Full Name dachsous 1 (Drosophila)
Calculated molecular weight 346 kDa
Observed molecular weight~350 kDa
GenBank accession numberNM_003737
Gene Symbol DCHS1
Gene ID (NCBI) 8642
RRIDAB_3669648
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCHS1 is one of two known pathogenic genes associated with mitral valve prolapse (MVP) , a common cardiac valve disease (PMID: 33225636). Dchs1 and Fat4 function as a ligand-receptor pair during murine development and these genes are required in multiple organs (PMID: 21303848).

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