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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse placenta tissue using DCDC2 Polyclonal antibody (26978-1-AP)

    WB analysis of mouse placenta using 26978-1-AP (same clone as 26978-1-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 26978-1-AP (DCDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DCDC2 Polyclonal antibody (26978-1-AP)

    WB analysis of HepG2 using 26978-1-AP (same clone as 26978-1-PBS)

    WB result of DCDC2 antibody (26978-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCDC2 transfected HepG2 cells. This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human Hepatocellular carcinoma using DCDC2 Polyclonal antibody (26978-1-AP)

    IHC staining of human Hepatocellular carcinoma using 26978-1-AP (same clone as 26978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular carcinoma slide using 26978-1-AP (DCDC2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using DCDC2 Polyclonal antibody (26978-1-AP)

    IF Staining of human liver cancer using 26978-1-AP (same clone as 26978-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using DCDC2 antibody (26978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DCDC2 Polyclonal antibody, PBS Only

DCDC2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 26978-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

DCDC2A, Doublecortin domain-containing protein 2, Protein RU2S, RU2, RU2S

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26978-1-PBS targets DCDC2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25577

Product name: Recombinant human DCDC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 351-476 aa of BC050704

Sequence: EDGEKANKDAEQKEDFSGMNGDLEEEGGREATDAPEQVEEILDHSEQQARPARVNGGTDEENGEELQQVNNELQLVLDKERKSQGAGSGQDEADVDPQRPPRPEVKITSPEENENNQQNKDYAAVA

相同性解析による交差性が予測される生物種
Full Name doublecortin domain containing 2
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC050704
Gene Symbol DCDC2
Gene ID (NCBI) 51473
RRIDAB_2880709
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHG0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCDC2 (Doublecortin domain-containing protein 2), also known as KIAA1154. DCDC2 is expressed in the central nervous system of adults and fetuses, especially in the olfactory cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus. DCDCDC2 protein contains two dicortical protein domains that bind to microtubules and enhance microtubule polymerization, which may play a role in neuronal migration and affect the signal transduction of primary cilia. In addition, DCDC2 also plays a role in inhibiting classical WNT signaling. DCDC2 may affect the development and function of the brain by affecting the migration of neurons and the signal conduction of primary cilia, and then it is related to reading ability (PMID: 21457949). The molecular weight of DCDC2 is 52 kDa, it also has another isoform with a molecular weight of 25 kDa.

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