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Validation Data Gallery

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  • Western Blot (WB) analysis of A-673 cells using DCC Recombinant antibody (85787-4-RR)

    WB analysis of A-673 using 85787-4-RR (same clone as 85787-4-PBS)

    A-673 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U266 cells using DCC Recombinant antibody (85787-4-RR)

    WB analysis of U266 using 85787-4-RR (same clone as 85787-4-PBS)

    U266 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02122-3

    Sandwich ELISA standard curve of MP02122-3

    Sandwich ELISA standard curve of MP02122-3, Human DCC Recombinant Matched Antibody Pair - PBS only. 85787-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2937. 85787-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP02122-3

    Sample test of MP02122-3

    The mean DCC concentration was determined to be 11.21 ng/mL in SH-SY5Y cell extract based on a 1.00 mg/mL extract load and 210.63 ng/mL in mouse brain tissue extract based on a 6.60 mg/mL extract load.

  • Cytometric bead array standard curve of MP02122-1

    Cytometric bead array standard curve of MP02122-1

    Cytometric bead array standard curve of MP02122-1, DCC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85787-4-PBS. Detection antibody: 85787-3-PBS. Standard: Eg2937. Range: 0.781-100 ng/mL.

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DCC Recombinant antibody, PBS Only (Capture)

DCC Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85787-4-PBS
Clone No.250085A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Colorectal cancer suppressor, CRC18, CRCR1, IGDCC1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85787-4-PBS targets DCC as part of a matched antibody pair:

MP02122-1: 85787-4-PBS capture and 85787-3-PBS detection (validated in Cytometric bead array)

MP02122-3: 85787-4-PBS capture and 85787-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DCC fusion protein Eg2937 相同性解析による交差性が予測される生物種
Full Name deleted in colorectal carcinoma
Calculated molecular weight158 kDa
Observed molecular weight180 kDa
GenBank accession numberNM_005215.3
Gene Symbol DCC
Gene ID (NCBI) 1630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43146
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody raised against a synthesized polypeptide of human DCC protein recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).