Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HEK-293T cells, HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32592-1-AP targets DCAF16 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36770 Product name: Recombinant human C4orf30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC101716 Sequence: MGPRNPSPDHLSESESEEEENISYLNESSGEEWDSSEEEDSMVPNLSPLESLAWQVKCLLKYSTTWKPLNPNSWLYHAKLLDPSTPVHILREIGLRLSHCSHCVPKLEPIPEWPPLASCGVPPFQKPLTSPSRLSRDHATLNGALQFATKQLSRTLSRATPIPEYLKQIPNSCVSGCCCGWLTKTVKETTRTEPINTTYSYTDFQKAVNKLLTASL 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 4 open reading frame 30 |
| Calculated molecular weight | 216 aa, 24 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC101716 |
| Gene Symbol | C4orf30 |
| Gene ID (NCBI) | 54876 |
| RRID | AB_3742666 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9NXF7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DCAF16 is a member of the DDB1-CUL4 associated factor (DCAF) family. It is a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serving as a crucial substrate recognition element. DCAF16 plays a central role in the ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DCAF16 antibody 32592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |