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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using DBNDD2 Polyclonal antibody (27623-1-AP)

    WB analysis of mouse brain using 27623-1-AP (same clone as 27623-1-PBS)

    mouse and rat brain tissues were subjected to SDS PAGE followed by western blot with 27623-1-AP (DBNDD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27623-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using DBNDD2 Polyclonal antibody (27623-1-AP)

    WB analysis of SH-SY5Y using 27623-1-AP (same clone as 27623-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27623-1-AP (DBNDD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DBNDD2 Polyclonal antibody (27623-1-AP)

    IHC staining of rat brain using 27623-1-AP (same clone as 27623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27623-1-AP (DBNDD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DBNDD2 Polyclonal antibody (27623-1-AP)

    IHC staining of rat brain using 27623-1-AP (same clone as 27623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27623-1-AP (DBNDD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27623-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27623-1-PBS targets DBNDD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBNDD2 fusion protein Ag26356 相同性解析による交差性が予測される生物種
Full Name dysbindin (dystrobrevin binding protein 1) domain containing 2
Calculated molecular weight 261 aa, 28 kDa
Observed molecular weight 28-29 kDa
GenBank accession numberBC001105
Gene Symbol DBNDD2
Gene ID (NCBI) 55861
RRIDAB_2880927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQY9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.