Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
22638-1-AP targets CCAR2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18374 Product name: Recombinant human DBC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 571-923 aa of BC018269 Sequence: EPEKEEAAKEEATKEEEAIKEEVVKEPKDEAQNEGPATESEAPLKEDGLLPKPLSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1967 |
| Calculated molecular weight | 923 aa, 103 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC018269 |
| Gene Symbol | CCAR2 |
| Gene ID (NCBI) | 57805 |
| RRID | AB_2879138 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N163 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCAR2 also known as DBC1 (Deleted in Breast Cancer 1), KIAA1967, NET35, and p30DBC, is a multifaceted nuclear protein that plays a critical role in various cellular processes, including transcriptional regulation, apoptosis (programmed cell death), DNA damage response, metabolism, and the maintenance of circadian rhythms. The protein is able to bind to RNA polymerase II complexes and exhibits enzyme inhibitor activity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| IHC protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| IP protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| WB protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''. | ||
Biochem Cell Biol Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1. | ||
Phytomedicine Genistein suppresses renal fibrosis in chronic kidney disease through regulation of the CCAR2/SIRT1/p53 signaling axis. | ||
Br J Pharmacol AdipoRon promotes amyloid-β clearance through enhancing autophagy via nuclear GAPDH-induced sirtuin 1 activation in Alzheimer's disease | ||
Elife Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression | ||
Free Radic Biol Med CircMETTL9 targets CCAR2 to induce neuronal oxidative stress and apoptosis via mitochondria-mediated pathways following traumatic brain injury |