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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of Raji using 67879-1-Ig (same clone as 67879-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of Daudi using 67879-1-Ig (same clone as 67879-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MOLT-4 cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of MOLT-4 using 67879-1-Ig (same clone as 67879-1-PBS)

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of Jurkat using 67879-1-Ig (same clone as 67879-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of HeLa using 67879-1-Ig (same clone as 67879-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAXX Monoclonal antibody (67879-1-Ig)

    IHC staining of human breast cancer using 67879-1-Ig (same clone as 67879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAXX Monoclonal antibody (67879-1-Ig)

    IHC staining of human breast cancer using 67879-1-Ig (same clone as 67879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DAXX Monoclonal antibody (67879-1-Ig)

    IF Staining of A431 using 67879-1-Ig (same clone as 67879-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAXX antibody (67879-1-Ig, Clone: 1F3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

DAXX Monoclonal antibody, PBS Only

DAXX Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67879-1-PBS
Clone No.1F3G9

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67879-1-PBS targets DAXX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14606

Product name: Recombinant human DAXX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC109074

Sequence: MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVD

相同性解析による交差性が予測される生物種
Full Name death-domain associated protein
Calculated molecular weight 740 aa, 81 kDa
Observed molecular weight100-130 kDa
GenBank accession numberBC109074
Gene Symbol DAXX
Gene ID (NCBI) 1616
RRIDAB_2918636
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UER7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

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