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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DARC Polyclonal antibody (55185-1-AP)

    WB analysis of K-562 using 55185-1-AP (same clone as 55185-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 55185-1-AP (DARC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55185-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DARC Polyclonal antibody (55185-1-AP)

    IHC staining of human kidney using 55185-1-AP (same clone as 55185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 55185-1-AP (DARC antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55185-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DARC Polyclonal antibody (55185-1-AP)

    IHC staining of human kidney using 55185-1-AP (same clone as 55185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 55185-1-AP (DARC antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55185-1-PBS in a different storage buffer formulation.

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DARC Polyclonal antibody, PBS Only

DARC Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55185-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55185-1-PBS targets DARC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Duffy blood group, chemokine receptor
Calculated molecular weight 36 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberNM_002036
Gene Symbol DARC
Gene ID (NCBI) 2532
RRIDAB_10838809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16570
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD234, also named as DARC, Duffy, FY and GPD, belongs to the G-protein coupled receptor Duffy family. It is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. This antibody is specific to CD234.