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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAPK3 Polyclonal antibody (30656-1-AP)

    WB analysis using 30656-1-AP (same clone as 30656-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30656-1-AP (DAPK3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DAPK3 Polyclonal antibody (30656-1-AP)

    IHC staining of human lung cancer using 30656-1-AP (same clone as 30656-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30656-1-AP (DAPK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DAPK3 Polyclonal antibody (30656-1-AP)

    IF Staining of A431 using 30656-1-AP (same clone as 30656-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAPK3 antibody (30656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

DAPK3 Polyclonal antibody, PBS Only

DAPK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30656-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ZIP kinase, DAP kinase 3, DAP like kinase, DAP-like kinase, Death-associated protein kinase 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30656-1-PBS targets DAPK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33051

Product name: Recombinant human DAPK3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 252-361 aa of NM_001348

Sequence: IRRLLVKDPKRRMTIAQSLEHSWIKAIRRRNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSYADFERFSKVLEEAAAAEEGLRELQRSRRLCHEDVEALAAIYE

相同性解析による交差性が予測される生物種
Full Name death-associated protein kinase 3
Calculated molecular weight 53 kDa
Observed molecular weight37, 52 kDa
GenBank accession numberNM_001348
Gene Symbol DAPK3
Gene ID (NCBI) 1613
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43293
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DAPK3, also named as ZIPK and Dlk, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. The calculated molecular weight of DAPK3 is 52 kDa. This antibody can recognize the 37 kDa isoform of target. ZIP kinase is a Serine/threonine kinase which acts as a positive regulator of apoptosis. ZIP kinasephosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. ZIP kinase regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

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