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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DAP3 Recombinant monoclonal antibody (86586-1-RR)

    WB analysis of HeLa using 86586-1-RR (same clone as 86586-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 86586-1-RR (DAP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86586-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DAP3 Recombinant monoclonal antibody (86586-1-RR)

    WB analysis using 86586-1-RR (same clone as 86586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86586-1-RR (DAP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86586-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DAP3 Recombinant monoclonal antibody (86586-1-RR)

    WB analysis using 86586-1-RR (same clone as 86586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86586-1-RR (DAP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86586-1-PBS in a different storage buffer formulation.

DAP3 Recombinant monoclonal antibody, PBS Only

DAP3 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86586-1-PBS
Clone No.251498H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

bMRP 10, DAP 3, DAP-3, Death-associated protein 3, MRP S29

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86586-1-PBS targets DAP3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0274

Product name: Recombinant human DAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 93-336 aa of BF220193

Sequence: TFSEACLMVRKPALELLHYLKNTSFAYPAIRYLLYGEKGTGKTLSLCHVIHFCAKQDWLILHIPDAHLWVKNCRDLLQSSYNKQRFDQPLEASTWLKNFKTTNERFLNQIKVQEKYVWNKRESTEKGSPLGEVVEQGITRVRNATDAVGIVLKELKRQSSLGMFHLLVAVDGINALWGRTTLKREDKSPIAPEELALVHNLRKMMKNDWHGGAIVSALSQTGSLFKPRKAYLPQELLGKEGFDA

相同性解析による交差性が予測される生物種
Full Name death associated protein 3
Calculated molecular weight 46 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBF220193
Gene Symbol DAP3
Gene ID (NCBI) 7818
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP51398
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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