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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using DAND5 Polyclonal antibody (22137-1-AP)

    WB analysis of human heart using 22137-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 22137-1-AP (DAND5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human heart using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human heart using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

DAND5 Polyclonal antibody

DAND5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22137-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CER2, Cerberus like protein 2, Cerl 2, CERL2, CKTSF1B3, COCO, CRL2, DAN domain family member 5, DAN domain family, member 5, DAND5, DANTE, GREM3, Gremlin 3, SP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22137-1-AP targets DAND5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17533

Product name: Recombinant human DAND5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-189 aa of BC113476

Sequence: MLLGQLSTLLCLLSGALPTGSGRPEPQSPRPQSWAAANQTWALGPGALPPLVPASALGSWKAFLGLQKARQLGMGRLQRGQDEVAAVTLPLNPQEVIQGMCKAVPFVQVFSRPGCSAIRLRNHLCFGHCSSLYIPGSDPTPLVLCNSCMPARKRWAPVVLWCLTGSSASRRRVKISTMLIEGCHCSPKA

相同性解析による交差性が予測される生物種
Full Name DAN domain family, member 5
Calculated molecular weight 189 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC113476
Gene Symbol DAND5
Gene ID (NCBI) 199699
RRIDAB_2879004
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DAND5 antibody 22137-1-APDownload protocol
IHC protocol for DAND5 antibody 22137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols