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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DACT1 Polyclonal antibody

DACT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27237-1-AP

Synonyms

DACT1, DAPPER, Dapper homolog 1, DAPPER1, DPR1, FRODO, HDPR1, HNG3, THYEX3

Validation Data Gallery

Applications:
  • WB analysis using 27237-1-AP

    WB analysis using 27237-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27237-1-AP (DACT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy DACT1 Ready-To-Use IHC Kit. DACT1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

27237-1-AP targets DACT1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DACT1 fusion protein Ag26096 相同性解析による交差性が予測される生物種
Full Name dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis)
Calculated molecular weight 90 kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_001079520
Gene symbol DACT1
Gene ID (NCBI) 51339
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DACT1 (Dapper/Frodo) is a member of the Dapper family, which includes three genes: DACT1, DACT2 and DACT3. As a tumor suppressor, DACT1 is downregulated in multiple tumor types, such as liver, colon and breast cancer . DACT1 is an important modulator of Wnt signaling, interacting with key components of the various Wnt transduction pathways. For instance, DACT1 binds to disheveled homolog 1 (Dvl1) to induce the degradation of Dvl1 and the subsequent inhibition of the Wnt/β-catenin pathway . (PMID: 34252542, PMID: 29456669)

Protocols

Product Specific Protocols
WB protocol for DACT1 antibody 27237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols