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DACH1 Polyclonal antibody

DACH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10914-1-AP

Synonyms

DACH, DACH1, Dachshund homolog 1, Dachshund-1



Tested Applications

Positive WB detected inH9C2 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman oesophagus cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

10914-1-AP targets DACH1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DACH1 fusion protein Ag1354 相同性解析による交差性が予測される生物種
Full Name dachshund homolog 1 (Drosophila)
Calculated molecular weight 79 kDa
Observed molecular weight 97-110 kDa
GenBank accession numberBC021219
Gene symbol DACH1
Gene ID (NCBI) 1602
RRIDAB_2230330
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

Protocols

Product Specific Protocols
WB protocol for DACH1 antibody 10914-1-APDownload protocol
IHC protocol for DACH1 antibody 10914-1-APDownload protocol
IF protocol for DACH1 antibody 10914-1-APDownload protocol
IP protocol for DACH1 antibody 10914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Single-cell analysis of early progenitor cells that build coronary arteries.

Authors - Tianying Su
humanIF

Nature

Excitation-induced ataxin-3 aggregation in neurons from patients with Machado-Joseph disease.

Authors - Koch Philipp P
mouseIF

Science

Breathing control center neurons that promote arousal in mice.

Authors - Kevin Yackle
humanIF

Cell Stem Cell

Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.

Authors - Carmen Lorenz
mouseIHC

Nat Cell Biol

A single-cell transcriptional roadmap for cardiopharyngeal fate diversification.

Authors - Wei Wang
humanWB

Nat Neurosci

Auto-attraction of neural precursors and their neuronal progeny impairs neuronal migration.

Authors - Julia Ladewig

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (11-02-2021)

This antibody works great for IF. It specifically marked DACH1-positive coronary vessel cells in mouse heart.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Heart