• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DAB1 Polyclonal antibody (31459-1-AP)

    WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DAB1 Polyclonal antibody (31459-1-AP)

    WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DAB1 Polyclonal antibody (31459-1-AP)

    IHC staining of rat brain using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DAB1 Polyclonal antibody (31459-1-AP)

    IHC staining of mouse brain using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DAB1 Polyclonal antibody (31459-1-AP)

    IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DAB1 Polyclonal antibody (31459-1-AP)

    IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DAB1 Polyclonal antibody (31459-1-AP)

    IF Staining of HepG2 using 31459-1-AP (same clone as 31459-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAB1 antibody (31459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

DAB1 Polyclonal antibody, PBS Only

DAB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31459-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Disabled homolog 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31459-1-PBS targets DAB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35376

Product name: Recombinant human DAB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 132-280 aa of NM_021080

Sequence: KEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLE

相同性解析による交差性が予測される生物種
Full Name disabled homolog 1 (Drosophila)
Calculated molecular weight64 kDa
Observed molecular weight45 kDa, 80 kDa
GenBank accession numberNM_021080
Gene Symbol DAB1
Gene ID (NCBI) 1600
RRIDAB_3742431
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDO75553
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Disabled-1 (Dab1) is an adaptor protein that is essential for neuronal migration and maturation in response to the extracellular protein Reelin. DAB1 protein docks to the intracellular part of the Reelin very low density lipoprotein receptor and apoE receptor type 2 and becomes tyrosine-phosphorylated following binding of Reelin to cortical neurons. The DAB1 protein contains a 180-amino acid N-terminal protein interaction/phosphotyrosine-binding (PTB) 1 domain that docks to the short cytoplasmic tail of the very low density lipoprotein receptor or apoE receptor type 2 at the level of NPXY motifs, with a preference for unphosphorylated motifs. Dab1 polypeptide bands ranging from 36 to 120 kDa have been identified in mouse embryonic brain, the 80-kDa Dab1 being the predominant form (PMID: 24313315).

||
New chat

Able™

正在加载，请稍候...