DAB kit (IHC)
Cat no : PR30018
Validation Data Gallery
Product Information
The HRP enzyme-labelled anti-mouse/rabbit secondary antibody binds specifically to the heavy and light chains of mouse and rabbit IgG. It passes through the diaminobenzidine (DAB) chromogenic solution to form a tan insoluble product. It is mainly used for immunohistochemical staining.
| Components | Size | ||||||
| Solution A | 20 mL | 100 mL | 1 L | 5 L | 10 L | 50 L | 100 L |
| Solution B | 1 mL | 5 mL | 0.05 L | 0.25 L | 0.5 L | 2.5 L | 5 L |
Storage
Store at 2-8℃ away from light, valid for 18 months.
Usage
1. DAB Staining Solution (freshly prepared as required): Mix solution A and solution B in a dilution ratio of 20:1, and use.
2. Add 50-100 uL of the above DAB staining solution to completely cover the sectioned tissue (if the tissue is large, the amount can be increased appropriately), incubate for 3-10 minutes, rinse with distilled water, and terminate the colour development.
Cautions
1. This product is used in immunohistochemistry experiments only.
2. Appropriate precautions should be taken to avoid the reagents' contact with skin and eyes.
3. This product is for scientific research use only. If you need to use it for diagnosis, please contact us for the record of product.
Cited in Article as
pr30018, DAB kit (IHC), Proteintech, IL, USA