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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAAM1 Monoclonal antibody (67287-1-Ig)

    WB analysis using 67287-1-Ig (same clone as 67287-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using DAAM1 Monoclonal antibody (67287-1-Ig)

    WB analysis of rat heart using 67287-1-Ig (same clone as 67287-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using DAAM1 Monoclonal antibody (67287-1-Ig)

    WB analysis of mouse heart using 67287-1-Ig (same clone as 67287-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using DAAM1 Monoclonal antibody (67287-1-Ig)

    IHC staining of human skin cancer using 67287-1-Ig (same clone as 67287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67287-1-Ig (DAAM1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

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DAAM1 Monoclonal antibody, PBS Only

DAAM1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67287-1-PBS
Clone No.3F11F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

3F11F11, Disheveled-associated activator of morphogenesis 1, FLJ41657, KIAA0666

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67287-1-PBS targets DAAM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DAAM1 fusion protein Ag6809 相同性解析による交差性が予測される生物種
Full Name dishevelled associated activator of morphogenesis 1
Calculated molecular weight 123 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC064999
Gene Symbol DAAM1
Gene ID (NCBI) 23002
RRIDAB_2882553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4D1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.