• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of NIH/3T3 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of NIH/3T3 using 66364-1-Ig (same clone as 66364-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of C6 using 66364-1-Ig (same clone as 66364-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of rat heart using 66364-1-Ig (same clone as 66364-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    FC experiment of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

D2HGDH Monoclonal antibody, PBS Only

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66364-1-PBS
Clone No.1E9B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

1E9B3, D2HGD, D-2-hydroxyglutarate dehydrogenase, mitochondrial, EC:1.1.99.39

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66364-1-PBS targets D2HGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5036

Product name: Recombinant human D2HGDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-286 aa of BC036604

Sequence: MLPRRPLAWPAWLLRGAPGAAGSWGRPVGPLARRGCCSAPGTPEVPLTRERYPVQRLPFSTVSKQDLAAFERIVPGGVVTDPEALQAPNVDWLRTLRGCSKVLLRPRTSEEVSHILRHCHERNLAVNPQGGNTGMVGGSVPVFDEIILSTARMNRVLSFHSVSGILVCQAGCVLEELSRYVEERDFIMPLDLGAKGSCHIGGNVATNAGGLRFLRYGSLHGTVLGLEVVLADGTVLDCLTSLRKDNTGYDLKQLFIGSEGTLGIITTVSILCPPKPRAVNVAFLGC

相同性解析による交差性が予測される生物種
Full Name D-2-hydroxyglutarate dehydrogenase
Calculated molecular weight 521 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC036604
Gene Symbol D2HGDH
Gene ID (NCBI) 728294
RRIDAB_2881744
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8N465
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).

||
New chat Able
正在加载，请稍候...