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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 80 Recombinant monoclonal antibody (86313-1-RR)

    WB analysis using 86313-1-RR (same clone as 86313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86313-1-RR (Cytokeratin 80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using Cytokeratin 80 Recombinant monoclonal antibody (86313-1-RR)

    IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using Cytokeratin 80 Recombinant monoclonal antibody (86313-1-RR)

    IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Cytokeratin 80 Recombinant monoclonal antibody (86313-1-RR)

    IF Staining of human breast cancer using 86313-1-RR (same clone as 86313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Cytokeratin 80 Recombinant monoclonal antibody (86313-1-RR)

    IF Staining of human breast cancer using 86313-1-RR (same clone as 86313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

Cytokeratin 80 Recombinant monoclonal antibody, PBS Only

Cytokeratin 80 Uni-rAb® Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 86313-1-PBS
Clone No.250883H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

KRT80, CK 80, CK-80, Cytokeratin-80, K80

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86313-1-PBS targets Cytokeratin 80 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name keratin 80
Calculated molecular weight 422 aa, 47 kDa
Observed molecular weight50 kDa
GenBank accession numberBC065180
Gene Symbol KRT80
Gene ID (NCBI) 144501
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ6KB66
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.

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