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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis using 68627-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of A549 using 68627-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of rat colon using 68627-1-Ig

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of mouse colon using 68627-1-Ig

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human colon using 68627-1-Ig

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human tonsillitis using 68627-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human colon using 68627-1-Ig

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human tonsillitis using 68627-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 8 Monoclonal antibody

Cytokeratin 8 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68627-1-Ig
Clone No.1F2H5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, rat colon tissue, mouse colon tissue, T-47D cells, SK-BR-3 cells, SW480 cells, HT-29 cells
Positive IHC detected inhuman colon tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68627-1-Ig targets Cytokeratin 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0488

Product name: Recombinant human KRT8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-483 aa of BC000654

Sequence: MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

相同性解析による交差性が予測される生物種
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight50-52 kDa
GenBank accession numberBC000654
Gene Symbol Cytokeratin 8
Gene ID (NCBI) 3856
RRIDAB_3085319
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP05787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Protocols

Product Specific Protocols
IHC protocol for Cytokeratin 8 antibody 68627-1-IgDownload protocol
WB protocol for Cytokeratin 8 antibody 68627-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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