Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)
mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 17391-1-AP (same clone as 17391-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
WB analysis of rat skin using 17391-1-AP (same clone as 17391-1-PBS)
rat skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human tonsil using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human tonsil using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus using 17391-1-AP (same clone as 17391-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 17391-1-AP (same clone as 17391-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 17391-1-AP (same clone as 17391-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.
×
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Cytokeratin 6B Polyclonal antibody, PBS Only

Cytokeratin 6B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 17391-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17391-1-PBS targets Cytokeratin 6B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11288 Product name: Recombinant human KRT6B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 214-564 aa of BC034535 Sequence: FEQYINNLRRQLDNIVGERGRLDSELRNMQDLVEDLKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQITAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRATGGGLSSVGGGSSTIKYTTTSSSSRKSYKH 相同性解析による交差性が予測される生物種
Full Name	keratin 6B
Calculated molecular weight	564 aa, 60 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC034535
Gene Symbol	Cytokeratin 6B
Gene ID (NCBI)	3854
RRID	AB_2134318
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04259
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)

WB analysis of A431 using 17391-1-AP (same clone as 17391-1-PBS)

WB analysis of rat skin using 17391-1-AP (same clone as 17391-1-PBS)

IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)

IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)

IHC staining of human breast cancer using 17391-1-AP (same clone as 17391-1-PBS)