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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5 Recombinant antibody (82548-7-RR)

    IHC staining of human lung cancer using 82548-7-RR

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 82548-7-RR (KRT5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5 Recombinant antibody (82548-7-RR)

    IHC staining of human lung cancer using 82548-7-RR

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 82548-7-RR (KRT5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 5 Recombinant antibody (82548-7-RR)

    IF Staining of A431 using 82548-7-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KRT5 antibody (82548-7-RR, Clone: 4N17 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 5 Recombinant antibody (82548-7-RR)

    IF Staining of HeLa using 82548-7-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT5 antibody (82548-7-RR, Clone: 4N17 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Cytokeratin 5 Recombinant monoclonal antibody

Cytokeratin 5 Uni-rAb® Recombinant Antibody for IF, IHC, ELISA
Cat No. 82548-7-RR
Clone No.4N17

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, ELISA

Synonyms

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman lung cancer tissue, human Mesothelioma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82548-7-RR targets Cytokeratin 5 in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24184

Product name: Recombinant human KRT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 316-491 aa of BC024292

Sequence: THVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNI

相同性解析による交差性が予測される生物種
Full Name keratin 5
Calculated molecular weight 590 aa, 62 kDa
GenBank accession numberBC024292
Gene Symbol Cytokeratin 5
Gene ID (NCBI) 3852
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP13647
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 5 antibody 82548-7-RRDownload protocol
IHC protocol for Cytokeratin 5 antibody 82548-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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