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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human tonsillitis using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human tonsillitis using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 5/6 Monoclonal antibody (68295-1-Ig)

    IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

Cytokeratin 5/6 Monoclonal antibody, PBS Only

Cytokeratin 5/6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 68295-1-PBS
Clone No.1F9E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

KRT6B, 1F9E1, CK 6B, CK5/6, CK6B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68295-1-PBS targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11288

Product name: Recombinant human KRT6B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 214-564 aa of BC034535

Sequence: FEQYINNLRRQLDNIVGERGRLDSELRNMQDLVEDLKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQITAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRATGGGLSSVGGGSSTIKYTTTSSSSRKSYKH

相同性解析による交差性が予測される生物種
Full Name keratin 6B
Calculated molecular weight 564 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC034535
Gene Symbol Cytokeratin 6B
Gene ID (NCBI) 3854
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP04259
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.

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