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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    WB analysis of A431 using 16572-1-AP (same clone as 16572-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human bladder tissue using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    WB analysis of human bladder using 16572-1-AP (same clone as 16572-1-PBS)

    human bladder tissue were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    IHC staining of human bowen disease using 16572-1-AP (same clone as 16572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    IHC staining of human bowen disease using 16572-1-AP (same clone as 16572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    IF Staining of mouse skin using 16572-1-AP (same clone as 16572-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 4 Polyclonal antibody (16572-1-AP)

    IF Staining of A431 using 16572-1-AP (same clone as 16572-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

Cytokeratin 4 Polyclonal antibody, PBS Only

Cytokeratin 4 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 16572-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16572-1-PBS targets Cytokeratin 4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9854

Product name: Recombinant human KRT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 184-534 aa of BC042174

Sequence: NLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGDNLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELARMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSSGFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR

相同性解析による交差性が予測される生物種
Full Name keratin 4
Calculated molecular weight 534 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC042174
Gene Symbol Cytokeratin 4
Gene ID (NCBI) 3851
RRIDAB_2134041
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19013
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.

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