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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using Cytokeratin 28 Recombinant monoclonal antibody (86434-1-RR)

    WB analysis of mouse skin using 86434-1-RR (same clone as 86434-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 86434-1-RR (KRT28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86434-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Cytokeratin 28 Recombinant monoclonal antibody (86434-1-RR)

    IF Staining of human colon cancer using 86434-1-RR (same clone as 86434-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 28 antibody (86434-1-RR, Clone: 251112E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86434-1-PBS in a different storage buffer formulation.

Cytokeratin 28 Recombinant monoclonal antibody, PBS Only

Cytokeratin 28 Uni-rAb® Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 86434-1-PBS
Clone No.251112E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

KRT28, CK 28, K25D, K28, Keratin 25D

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86434-1-PBS targets Cytokeratin 28 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name keratin 28
Calculated molecular weight 464 aa, 51 kDa
Observed molecular weight51-60 kDa
GenBank accession numberBC148794
Gene Symbol KRT28
Gene ID (NCBI) 162605
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ7Z3Y7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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