Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis using 82428-1-RR (same clone as 82428-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 82428-1-RR (same clone as 82428-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
IF Staining of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
FC experiment of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)
1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.
×
1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

Cytokeratin 20 Recombinant monoclonal antibody, PBS Only

Cytokeratin 20 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Cat No. 82428-1-PBS

Clone No.2H10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82428-1-PBS targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag11308 Product name: Recombinant human KRT20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-424 aa of BC031559 Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI 相同性解析による交差性が予測される生物種
Full Name	keratin 20
Calculated molecular weight	424 aa, 48 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC031559
Gene Symbol	Cytokeratin 20
Gene ID (NCBI)	54474
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35900
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 82428-1-RR (same clone as 82428-1-PBS)

WB analysis of COLO 320 using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)

IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)

IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)