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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    WB analysis using 86250-1-RR (same clone as 86250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    WB analysis of A549 using 86250-1-RR (same clone as 86250-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cytokeratin 19 Recombinant antibody (86250-1-RR)

    IF Staining of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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Cytokeratin 19 Recombinant antibody, PBS Only

Cytokeratin 19 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86250-1-PBS
Clone No.250894B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86250-1-PBS targets Cytokeratin 19 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC002539
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08727
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.