Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis using 86250-1-RR (same clone as 86250-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 86250-1-RR (same clone as 86250-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IF Staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IF Staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
FC experiment of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cytokeratin 19 Recombinant antibody (86250-1-RR, Clone:250894B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.
×
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cytokeratin 19 Recombinant antibody (86250-1-RR, Clone:250894B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

Cytokeratin 19 Recombinant monoclonal antibody, PBS Only

Cytokeratin 19 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Cat No. 86250-1-PBS

Clone No.250894B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86250-1-PBS targets Cytokeratin 19 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	keratin 19
Calculated molecular weight	44 kDa
Observed molecular weight	44 kDa
GenBank accession number	BC002539
Gene Symbol	Cytokeratin 19
Gene ID (NCBI)	3880
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08727
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 86250-1-RR (same clone as 86250-1-PBS)

WB analysis of A549 using 86250-1-RR (same clone as 86250-1-PBS)

IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)