Validation Data Gallery
Tested Applications
| Positive WB detected in | HaCaT cells, mouse skin tissue |
| Positive IHC detected in | mouse skin tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86761-3-RR targets Cytokeratin 15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 15 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 46-50 kDa |
| GenBank accession number | BC002641 |
| Gene Symbol | KRT15 |
| Gene ID (NCBI) | 3866 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19012 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytokeratin 15 (CK15, encoded by KRT15) is a type I intermediate filament protein belonging to the keratin family. It is predominantly expressed in the basal and suprabasal layers of stratified epithelia, with particularly strong expression in the bulge region of the hair follicle, a well-characterized niche for epithelial stem cells. CK15 serves as a reliable marker for quiescent stem cells in various tissues, including the skin, mammary gland, and prostate, and its expression is often maintained during early stages of epithelial differentiation. In pathological contexts, CK15 is utilized as an immunohistochemical marker to distinguish basal cell carcinomas from other cutaneous neoplasms and to identify subsets of aggressive breast and prostate cancers. Its role in maintaining stem cell properties and tissue homeostasis makes it a valuable tool in both developmental biology and diagnostic pathology.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cytokeratin 15 antibody 86761-3-RR | Download protocol |
| WB protocol for Cytokeratin 15 antibody 86761-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |