• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cytokeratin 10 Recombinant monoclonal antibody (86324-1-RR)

    WB analysis using 86324-1-RR (same clone as 86324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86324-1-RR (KRT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 10 Recombinant monoclonal antibody (86324-1-RR)

    IHC staining of human skin cancer using 86324-1-RR (same clone as 86324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 10 Recombinant monoclonal antibody (86324-1-RR)

    IHC staining of human skin cancer using 86324-1-RR (same clone as 86324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using Cytokeratin 10 Recombinant monoclonal antibody (86324-1-RR)

    IF Staining of mouse skin using 86324-1-RR (same clone as 86324-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using Cytokeratin 10 Recombinant monoclonal antibody (86324-1-RR)

    IF Staining of mouse skin using 86324-1-RR (same clone as 86324-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

Cytokeratin 10 Recombinant monoclonal antibody, PBS Only

Cytokeratin 10 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86324-1-PBS
Clone No.250893H2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

KRT10, CK 10, CK10, CK-10, Cytokeratin,Keratin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86324-1-PBS targets Cytokeratin 10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name keratin 10
Calculated molecular weight 584 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC034697
Gene Symbol Cytokeratin 10
Gene ID (NCBI) 3858
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP13645
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cytokeratin 10 (CK-10, K10, KRT10) is a type I (acidic) cytokeratin and part of the intermediate filament protein superfamily. It is expressed in keratinized stratified epithelium, suprabasal cell layers (including the stratum corneum), and differentiated areas of highly differentiated squamous cell carcinomas. CK-10 plays a role in maintaining the structural integrity of epithelial cells and is involved in differentiation, tissue specialization, and epidermal barrier establishment. Mutations in the KRT10 gene are associated with epidermolytic hyperkeratosis, and its expression is linked to the terminal differentiation of epidermal cells . CK-10 is also used as a marker for tissue differentiation and characterization of malignant tumors in surgical pathology.

||
New chat Able
正在加载，请稍候...