• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cystatin B Monoclonal antibody

Cystatin B Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G9B8

Cat no : 66812-1-Ig

Synonyms

CPI B, CST6, CSTB, Cystatin B, cystatin B (stefin B), EPM1, PME, Stefin B, STFB

Validation Data Gallery

Applications:
  • WB analysis of U-937 using 66812-1-Ig

    WB analysis of U-937 using 66812-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of human saliva using 66812-1-Ig

    WB analysis of human saliva using 66812-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 66812-1-Ig

    WB analysis of THP-1 using 66812-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 66812-1-Ig

    IF Staining of A431 using 66812-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cystatin B antibody (66812-1-Ig, Clone: 2G9B8 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU-937 cells, human saliva tissue, THP-1 cells
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CSTB Ready-To-Use IHC Kit. CSTB primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66812-1-Ig targets Cystatin B in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cystatin B fusion protein Ag21291 相同性解析による交差性が予測される生物種
Full Name cystatin B (stefin B)
Calculated molecular weight 11 kDa
Observed molecular weight 11-14 kDa
GenBank accession numberBC003370
Gene symbol CSTB
Gene ID (NCBI) 1476
RRIDAB_2882155
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Protocols

Product Specific Protocols
WB protocol for Cystatin B antibody 66812-1-IgDownload protocol
IF protocol for Cystatin B antibody 66812-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols