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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of human saliva using 10823-1-AP (same clone as 10823-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of HepG2 using 10823-1-AP (same clone as 10823-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    IP experiment of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    IP result of anti-Cystatin B (IP:10823-1-AP, 4ug; Detection:10823-1-AP 1:4000) with MCF-7 cells lysate 1320ug. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cystatin B Polyclonal antibody (10823-1-AP)

    IHC staining of human colon using 10823-1-AP (same clone as 10823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Cystatin B Polyclonal antibody (10823-1-AP)

    IHC staining of human stomach using 10823-1-AP (same clone as 10823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    IF Staining of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    IF Staining of HepG2 using 10823-1-AP (same clone as 10823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cystatin B Polyclonal antibody, PBS Only

Cystatin B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10823-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CSTB, EPM1, Cystatin-B, CST6, CPI-B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10823-1-PBS targets Cystatin B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1262

Product name: Recombinant human CSTB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-98 aa of BC003370

Sequence: MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF

相同性解析による交差性が予測される生物種
Full Name cystatin B (stefin B)
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC003370
Gene Symbol Cystatin B
Gene ID (NCBI) 1476
RRIDAB_2086100
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04080
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

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