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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyp2c7 Polyclonal antibody

Cyp2c7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55452-1-AP

Synonyms

Cyp2c39, Cyp2c7

Validation Data Gallery

Applications:
  • WB analysis of rat heart using 55452-1-AP

    WB analysis of rat heart using 55452-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 55452-1-AP (Cyp2c7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 55452-1-AP

    IHC staining of rat liver using 55452-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55452-1-AP (Cyp2c7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 55452-1-AP

    IHC staining of rat liver using 55452-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55452-1-AP (Cyp2c7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat heart tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55452-1-AP targets Cyp2c7 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily c, polypeptide 7
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberNM_017158
Gene symbol Cyp2c7
Gene ID (NCBI) 29298
RRIDAB_2881339
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cyp2c7 antibody 55452-1-APDownload protocol
IHC protocol for Cyp2c7 antibody 55452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols