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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse stomach tissue using Cyclon/CCDC86 Polyclonal antibody (14947-1-AP)

    WB analysis of mouse stomach using 14947-1-AP (same clone as 14947-1-PBS)

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Cyclon/CCDC86 Polyclonal antibody (14947-1-AP)

    WB analysis of human heart using 14947-1-AP (same clone as 14947-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using Cyclon/CCDC86 Polyclonal antibody (14947-1-AP)

    WB analysis of human liver using 14947-1-AP (same clone as 14947-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Cyclon/CCDC86 Polyclonal antibody (14947-1-AP)

    WB analysis of HepG2 using 14947-1-AP (same clone as 14947-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using Cyclon/CCDC86 Polyclonal antibody (14947-1-AP)

    WB analysis of human stomach using 14947-1-AP (same clone as 14947-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Cyclon/CCDC86 Polyclonal antibody (14947-1-AP)

    IF Staining of A549 using 14947-1-AP (same clone as 14947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cyclon/CCDC86 antibody (14947-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

Cyclon/CCDC86 Polyclonal antibody, PBS Only

Cyclon/CCDC86 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 14947-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14947-1-PBS targets Cyclon/CCDC86 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6754

Product name: Recombinant human CCDC86 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-360 aa of BC001378

Sequence: MDTPLRRSRRLGGLRPESPESLTSVSRTRRALVEFESNPEETREPGSPPSVQRAGLGSPERPPKTSPGSPRLQQGAGLESPQGQPEPGAASPQRQQDLHLESPQRQPEYSPESPRCQPKPSEEAPKCSQDQGVLASELAQNKEELTPGAPQHQLPPVPGSPEPYPGQQAPGPEPSQPLLELTPRAPGSPRGQHEPSKPPPAGETVTGGFGAKKRKGSSSQAPASKKLNKEELPVIPKGKPKSGRVWKDRSKKRFSQMLQDKPLRTSWQRKMKERQERKLAKDFARHLEEEKERRRQEKKQRRAENLKRRLENERKAEVVQVIRNPAKLKRAKKKQLRSIEKRDTLALLQKQPPQQPAAKI

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 86
Calculated molecular weight 40 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC001378
Gene Symbol CCDC86
Gene ID (NCBI) 79080
RRIDAB_2072318
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6F5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCDC86 (Coiled-coil domain-containing protein 86), also known as Cyclon (cytokine-induced protein with coiled-coil domain) ) is known for its expression in leukocytes in mice, where it regulates the immune response. Cyclon/CCDC86 regulates the apoptosis of T-cells upon activation, a process known as an activationinduced cellular death (AICD), by regulating the expression of the death receptor Fas on T-cells (PMID: 23439384). CCDC86 depletion led to a novel phenotype in which Ki-67 and nucleolin could still accumulate at the chromosome periphery but also formed abnormal cytoplasmic NDF-like foci (PMID: 36695333). The detected molecular weights (MWs) of Cyclon on SDS-PAGE (human Cyclon: 65 kDa; mouse Cyclon: 75 kDa) were higher than calculated MWs (human Cyclon: 40.2 kDa; mouse Cyclon: 46.5 kDa), suggesting possible modifications of Cyclon (PMID: 17300783).

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