• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cyclin T1 Recombinant antibody (84980-1-RR)

    WB analysis using 84980-1-RR (same clone as 84980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84980-1-RR (Cyclin T1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84980-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cyclin T1 Recombinant antibody (84980-1-RR)

    FC experiment of MCF-7 using 84980-1-RR (same clone as 84980-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84980-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using Cyclin T1 Recombinant antibody (84980-1-RR)

    FC experiment of U2OS using 84980-1-RR (same clone as 84980-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84980-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Cyclin T1 Recombinant antibody, PBS Only

Cyclin T1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84980-1-PBS
Clone No.242256C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

CCNT1, Cyclin-T1, Cyclin-T, Cyclin T, CCNT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84980-1-PBS targets Cyclin T1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cyclin T1
Calculated molecular weight 81 kDa
Observed molecular weight 75-81 kDa
GenBank accession numberNM_001240
Gene Symbol Cyclin T1
Gene ID (NCBI) 904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60563
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CCNT1, also named as Cyclin T1 and CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.