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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of rat testis tissue using Cyclin E1 Recombinant antibody (82700-9-RR)

    IHC staining of rat testis using 82700-9-RR (same clone as 82700-9-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using Cyclin E1 Recombinant antibody (82700-9-RR)

    IHC staining of rat testis using 82700-9-RR (same clone as 82700-9-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Cyclin E1 Recombinant antibody (82700-9-RR)

    IHC staining of mouse testis using 82700-9-RR (same clone as 82700-9-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Cyclin E1 Recombinant antibody (82700-9-RR)

    IHC staining of mouse testis using 82700-9-RR (same clone as 82700-9-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cyclin E1 Recombinant antibody (82700-9-RR)

    IF Staining of HepG2 using 82700-9-RR (same clone as 82700-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclin E1 Recombinant antibody (82700-9-RR)

    IF Staining of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Cyclin E1 Recombinant antibody (82700-9-RR)

    FC experiment of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cyclin E1 Recombinant antibody (82700-9-RR)

    FC experiment of MCF-7 using 82700-9-RR (same clone as 82700-9-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82700-9-PBS

    Affinity and Kinetic Characterization of 82700-9-PBS

    Biolayer interferometry (BLl) kinetic assay of 82700-9-PBS against Human Cyclin E1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 82700-9-RR

    Affinity and Kinetic Characterization of 82700-9-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-9 -RR against Human Cyclin E were performed. The affinity constant is below 1 pM.

Cyclin E1 Recombinant monoclonal antibody, PBS Only

Cyclin E1 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82700-9-PBS
Clone No.240136H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CCNE1, 240136H5, CCNE, Cyclin E, G1/S specific cyclin E1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82700-9-PBS targets Cyclin E1 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2110

Product name: Recombinant human Cyclin E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 111-410 aa of BC035498

Sequence: ANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA

相同性解析による交差性が予測される生物種
Full Name cyclin E1
Calculated molecular weight 410 aa, 47 kDa
GenBank accession numberBC035498
Gene Symbol Cyclin E1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP24864
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).