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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin D2 Monoclonal antibody (67048-1-Ig)

    WB analysis using 67048-1-Ig (same clone as 67048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Cyclin D2 Monoclonal antibody (67048-1-Ig)

    WB analysis of NIH/3T3 using 67048-1-Ig (same clone as 67048-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67048-1-PBS in a different storage buffer formulation.

Cyclin D2 Monoclonal antibody, PBS Only

Cyclin D2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67048-1-PBS
Clone No.1A12A8

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67048-1-PBS targets Cyclin D2 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25190

Product name: Recombinant human CCND2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-289 aa of BC010958

Sequence: MELLCHEVDPVRRAVRDRNLLRDDRVLQNLLTIEERYLPQCSYFKCVQKDIQPYMRRMVATWMLEVCEEQKCEEEVFPLAMNYLDRFLAGVPTPKSHLQLLGAVCMFLASKLKETSPLTAEKLCIYTDNSIKPQELLEWELVVLGKLKWNLAAVTPHDFIEHILRKLPQQREKLSLIRKHAQTFIALCATDFKFAMYPPSMIATGSVGAAICGLQQDEEVSSLTCDALTELLAKITNTDVDCLKACQEQIEAVLLNSLQQYRQDQRDGSKSEDELDQASTPTDVRDIDL

相同性解析による交差性が予測される生物種
Full Name cyclin D2
Calculated molecular weight 32 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC010958
Gene Symbol Cyclin D2
Gene ID (NCBI) 894
RRIDAB_2882361
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP30279
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.