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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin B1 Recombinant antibody (85895-3-RR)

    WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85895-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin B1 Recombinant antibody (85895-3-RR)

    WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85895-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85895-3-RR

    Affinity and Kinetic Characterization of 85895-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85895-3-RR against Human Cyclin B1 were performed. The affinity constant is 2.70 nM.

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Cyclin B1 Recombinant antibody, PBS Only

Cyclin B1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85895-3-PBS
Clone No.250227C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85895-3-PBS targets Cyclin B1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin B1 fusion protein Ag29426 相同性解析による交差性が予測される生物種
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC006510
Gene Symbol Cyclin B1
Gene ID (NCBI) 891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14635
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.