Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

IP result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.4 ug Cyclin B1 Polyclonal antibody (55004-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.