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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin A2 Recombinant antibody, PBS Only

Cyclin A2 Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C23

Cat no : 82148-1-PBS

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, cyclin A2

Validation Data Gallery

Applications:
  • WB analysis using 82148-1-RR (same clone as 82148-1-PBS)

    WB analysis using 82148-1-RR (same clone as 82148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82148-1-RR (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    IP experiment of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    IP result of anti-Cyclin A2 (IP:82148-1-RR, 4ug; Detection:82148-1-RR 1:3000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 82148-1-RR (same clone as 82148-1-PBS)

    IHC staining of rat colon using 82148-1-RR (same clone as 82148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IHC staining of mouse colon using 82148-1-RR (same clone as 82148-1-PBS)

    IHC staining of mouse colon using 82148-1-RR (same clone as 82148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IF Staining of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    IF Staining of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin A2 antibody (82148-1-RR, Clone: 1C23 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy CCNA2 Ready-To-Use IHC Kit. CCNA2 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

82148-1-PBS targets Cyclin A2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin A2 fusion protein Ag12765 相同性解析による交差性が予測される生物種
Full Name cyclin A2
Calculated molecular weight 432 aa, 49 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC104783
Gene symbol CCNA2
Gene ID (NCBI) 890
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.