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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Connexin 45 Polyclonal antibody (22184-1-AP)

    WB analysis using 22184-1-AP (same clone as 22184-1-PBS)

    mouse heart tissue was subjected to SDS PAGE followed by western blot with 22184-1-AP (Connexin 45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Connexin 45 Polyclonal antibody (22184-1-AP)

    IHC staining of mouse brain using 22184-1-AP (same clone as 22184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22184-1-AP (Connexin 45 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22184-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Connexin 45 Polyclonal antibody, PBS Only

Connexin 45 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22184-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Connexin-45, Cx45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22184-1-PBS targets Connexin 45 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin 45 fusion protein Ag16742 相同性解析による交差性が予測される生物種
Full Name gap junction protein, gamma 1, 45kDa
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC096213
Gene Symbol Connexin-45
Gene ID (NCBI) 10052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36383
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Connexin 45 (Cx45), also known as Gap junction gamma protein (GJC1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. A loss-of-function mutation in Cx45 contributing to Atrial fibrillation (AF) and other cardiac arrhythmias (PMID: 33429106).