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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Connexin 43 Recombinant antibody (80543-1-RR)

    WB analysis using 80543-1-RR (same clone as 80543-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80543-1-RR (Connexin 43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Connexin 43 Recombinant antibody (80543-1-RR)

    IF Staining of HeLa using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80543-1-RR

    Affinity and Kinetic Characterization of 80543-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80543-1-RR against Human Connexin 43 were performed. The affinity constant is 5.19 nM.

Connexin 43 Recombinant monoclonal antibody, PBS Only

Connexin 43 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 80543-1-PBS
Clone No.3O6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Connexin-43, GJA1, 3O6, CX43, Gap junction alpha 1 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80543-1-PBS targets Connexin 43 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25533

Product name: Recombinant human Connexin 43 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 232-382 aa of BC026329

Sequence: FFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

相同性解析による交差性が予測される生物種
Full Name gap junction protein, alpha 1, 43kDa
Calculated molecular weight 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC026329
Gene Symbol Connexin-43
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17302
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).