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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Complement factor H Polyclonal antibody (12748-1-AP)

    WB analysis using 12748-1-AP (same clone as 12748-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using Complement factor H Polyclonal antibody (12748-1-AP)

    WB analysis of human blood using 12748-1-AP (same clone as 12748-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using Complement factor H Polyclonal antibody (12748-1-AP)

    IP experiment of human plasma using 12748-1-AP (same clone as 12748-1-PBS)

    IP result of anti-Complement factor H (IP:12748-1-AP, 4ug; Detection:12748-1-AP 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IF Staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Complement factor H Polyclonal antibody (12748-1-AP)

    IF Staining of HepG2 using 12748-1-AP (same clone as 12748-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

Complement factor H Polyclonal antibody, PBS Only

Complement factor H Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 12748-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12748-1-PBS targets Complement factor H in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3482

Product name: Recombinant human CFH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-349 aa of BC037285

Sequence: LPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAV

相同性解析による交差性が予測される生物種
Full Name complement factor H
Calculated molecular weight 1231 aa, 139 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC037285
Gene Symbol Complement factor H
Gene ID (NCBI) 3075
RRIDAB_10642946
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP08603
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).

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