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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Complement factor B Recombinant antibody (84101-5-RR)

    WB analysis using 84101-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84101-5-RR (Complement factor B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Complement factor B Recombinant antibody (84101-5-RR)

    IHC staining of mouse liver using 84101-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Complement factor B Recombinant antibody (84101-5-RR)

    IHC staining of mouse liver using 84101-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Complement factor B Recombinant antibody (84101-5-RR)

    IF Staining of HepG2 using 84101-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor B antibody (84101-5-RR, Clone: 241141A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 84101-5-RR

    Affinity and Kinetic Characterization of 84101-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84101-5-RR against Human Complement factor B were performed. The affinity constant is 2.27 nM.

Complement factor B Recombinant monoclonal antibody

Complement factor B Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84101-5-RR
Clone No.241141A8
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CFB, CFAB, C3/C5 convertase, BFD, BF

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse serum, rat serum, rat plasma, human plasma
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84101-5-RR targets Complement factor B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0226

Product name: Recombinant human CFB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 574-762 aa of BC007990

Sequence: DYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLG

相同性解析による交差性が予測される生物種
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93-100 kDa
GenBank accession numberBC007990
Gene Symbol Complement factor B
Gene ID (NCBI) 629
RRIDAB_3671666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP00751
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease that is associated with C3b and forms the alternative pathway for C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Protocols

Product Specific Protocols
IF protocol for Complement factor B antibody 84101-5-RRDownload protocol
IHC protocol for Complement factor B antibody 84101-5-RRDownload protocol
WB protocol for Complement factor B antibody 84101-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Lab Invest

Complement Component 3 Promotes Regeneration of Olfactory Receptor Neurons

Authors - Hiroki Kuwazoe
View Article
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