Product Information

Proteintech's Protease Inhibitor Cocktail contains a mixture of protease inhibitors including PMSF, E-64, Aprotinin, Bestatin, Leuptin, and Pepstatin. It mainly inhibits aminopeptidase, cysteine and serine proteases, serine/threonine and tyrosine proteases, aspartate proteases, thermophils, and other metalloendopeptidases. The cocktail can effectively prevent protein degredation during extraction or lysis of primary cells, mammalian cultured cells, animal tissues, plant tissues, yeast, or bacteria. It can be used for multiple applications including protein purification, western blotting, immunoprecipitation, immunofluoresence, immunohistochemistry and other studies.

Components

It contains a mixture of protease inhibitors including PMSF, E-64, Aprotinin, Bestatin, Leuptin, and Pepstatin. 

Storage

Store at -20°C for up to one year.

Usage

Dissolve well at room temperature and add to the lysate or sample solution at a dilution ratio of 1:100 or according to the needs of the experiment.

Notes

1. The protease content can differ across samples. Therefore, the amount of protease inhibitor cocktail can be adjusted according to the experimental situation.

2. This product is a highly concentrated solution. A small amount of precipitation may occur and, therefore, needs to be fully dissolved before use.

3. PMSF is very unstable in aqueous solution, so the cocktail should be added right before use.

4. For your safety and health, please wear a lab coat and disposable gloves.

Cited in Article as

pr20032, Common Protease Inhibitor Cocktail (100X), Proteintech, IL, USA